Bioactive, Human TIGIT Dimer, His-Avi Tag

Product CodeCSP-24028-03
Expression HostHEK293T
Verified ApplicationsELISA for TIGIT-specific antibody, Nectin-4 ligand protein, and CD155 ligand protein binding assays.
Suggested ApplicationsSPR & BLI for TIGIT-specific antibody, Nectin-4 ligand protein and CD155 receptor protein binding assays. Animal immunization, RUO.
PurityGreater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range Met22-Phe141

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Bioactivity – Antibody Binding
TIGIT His-Avi antibody binding
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Immobilized TIGIT-His-Avi dimer protein (Cat. No. CSP-24028-03) at 2 μg/mL (100 μL/well) can bind anti-human TIGIT monoclonal antibody with half maximal effective concentration (EC50) range of 6.2-24.8 ng/mL (QC tested).

Bioactivity – Ligand Binding
TIGIT His-Avi ligand binding
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Immobilized human Nectin-4 dimer protein (Cat. No. CSP-24016) at 5 μg/mL (100 μL/well) can bind TIGIT-His-Avi dimer protein (Cat. No. CSP-24028-03) with half maximal effective concentration (EC50) range of 0.88-3.53 μg/mL (QC tested).

SDS-PAGE
TIGIT His-Avi SDS PAGE
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MW: Molecular Weight marker reduced condition
NR: TIGIT dimer under non-reducing condition

Specifications

Protein Name: TIGIT

UniProt #: Q495A1

Predicted Molecular Weight: 60 kDa

SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing conditions is 50-60 kDa on SDS PAGE.

Protein Construct: TIGIT dimer protein (TIGIT protein dimer) contains a TIGIT extracellular domain (UniProt# Q495A1) with a tandem His-Avi tag at the C-terminus.

Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C

Human TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is a Type I transmembrane protein, also known as VSIG9 and VSTM3. TIGIT dimerization on the cell surface is a key mechanism in regulating immune responses. The recombinant TIGIT dimer protein is a cis-homodimer (CSP-24028-03) and contains a TIGIT extracellular domain (UniProt# Q495A1, amino acids Met22-Phe141) with a tandem His-Avi tag at the C-terminus. This protein is expressed in HEK293 cells. The TIGIT dimer-His-Avi protein is bioactive and can bind to Nectin-4 and CD155. It also binds to TIGIT-specific antibodies. This TIGIT dimer can be used as an antigen for in vitro assays and antibody screening, targeting more conformational epitopes.

Background

Human TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 (V-set and immunoglobulin domain-containing protein 9) and VSTM3 (V-set and transmembrane domain-containing protein 3). TIGIT is a type 1 membrane protein containing an immunoglobulin variable (Ig-V) domain, a transmembrane domain and cytoplasmic domain. TIGIT is an immune receptor present on peripheral memory and regulatory CD4+ T cells and natural killer (NK) cells. TIGIT binds to CD155 (the poliovirus receptor, PVR) with high affinity and binds to CD112 (PVRL2) with lower affinity. Nectin-4 is also a ligand for TIGIT. TIGIT’s action is highly dependent on its dimerization at the cell surface. TIGIT plays a role in immune suppression and is a therapeutic target for diseases where immune modulation is critical, including cancer and autoimmune diseases.

Alternate Names: VSIG9, VSTM3

Learn more about TIGIT