Bioactive, Human TIGIT Dimer, His Tag
| Product Code | CSP-24028-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for TIGIT-specific antibody and Nectin-4 ligand protein binding assays. |
| Suggested Applications | ELISA for TIGIT-specific antibody, Nectin-4 ligand protein, and CD155 receptor protein binding assays. SPR & BLI for TIGIT-specific antibody, Nectin-4 ligand protein, and CD155 receptor protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | M22-P141 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized TIGIT-His dimer protein (Cat. No. CSP-24028-01) at 2 μg/mL (100 μL/well) can bind anti-human TIGIT monoclonal antibody with half maximal effective concentration (EC50) range of 4.1-16.6 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: TIGIT dimer under non-reducing condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 and VSTM3. The recombinant TIGIT dimer protein (CSP-24028-01) is a cis-homodimer (cis-dimer) and contains a TIGIT extracellular domain (UniProt# Q495A1, amino acids Met22-Phe141) fused with a proprietary dimer motif followed by a His-tag at the C-terminus. This protein is expressed in HEK293T cells. The TIGIT-His dimer protein is bioactive and can bind to Nectin-4 and CD155. It also binds to TIGIT-specific antibodies. This TIGIT dimer can be used as an antigen for in vitro assays and antibody screening, targeting more conformational epitopes.
Protein Name: TIGIT
UniProt #: Q495A1
Predicted Molecular Weight: 42 kDa
SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing conditions is 40-70 kDa on SDS PAGE.
Protein Construct: TIGIT protein dimer contains a TIGIT extracellular domain (UniProt# Q495A1) with a His tag at the C-terminus.
Background
Human TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 (V-set and immunoglobulin domain-containing protein 9) and VSTM3 (V-set and transmembrane domain-containing protein 3). TIGIT is a type I membrane protein containing an immunoglobulin variable (Ig-V) domain, a transmembrane domain and cytoplasmic domain. TIGIT is an immune receptor present on peripheral memory and regulatory CD4+ T cells and natural killer (NK) cells. TIGIT binds to CD155 (the poliovirus receptor, PVR) with high affinity and binds to CD112 (PVRL2) with lower affinity. Nectin-4 is also a ligand for TIGIT.
Alternate Names: VSIG9, VSTM3