Bioactive, Recombinant Mouse IL-15Rα Protein Dimer, His Tag
| Product Code | CSP-25158-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for IL-15Rα-specific antibody and interleukin 15 (IL-15) ligand protein binding assays. |
| Suggested Applications | SPR & BLI for IL-15Rα-specific antibody and IL-15 protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | G33-K205 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized mouse IL-15Rα protein dimer, His-tag (CSP-25158-01) at 2 μg/mL (100 μL/well) can bind anti-mouse IL15Rα monoclonal antibody with half maximal effective concentration (EC50) range of 12-47.8 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized mouse IL-15 protein at 2 μg/mL (100 μL/well) can bind mouse IL-15Rα protein dimer, His-tag (CSP-25158-01) with half maximal effective concentration (EC50) range of 26.1-104.4 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: IL-15Rα dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Mouse interleukin 15 receptor alpha subunit (IL-15Rα) is a transmembrane cytokine receptor. The recombinant IL-15Rα protein dimer (CSP-25158-01) is a cis-homodimer (cis-dimer) and contains an IL-15Rα extracellular domain (UniProt# Q60819, amino acids Gly33-Lys205) fused with a proprietary cis-dimer motif followed by a His-tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant mouse IL-15Rα dimer protein is bioactive and can bind to interleukin 15 (IL-15). It also binds IL-15Rα -specific antibodies. This IL-15Rα protein dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IL-15Rα
UniProt #: Q60819
Predicted Molecular Weight: 53 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reduced condition is 120-190 kDa on SDS PAGE.
Protein Construct: IL-15Rα protein dimer contains an IL-15Rα extracellular domain (UniProt# Q60819) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Interleukin 15 receptor alpha subunit (IL-15Rα) is a transmembrane cytokine receptor. IL-15Rα is also known as IL-15Ra, IL15Ra, cluster of differentiation 215 (CD215), and interleukin 15 receptor subunit alpha. IL-15Rα contains an extracellular domain with a single sushi domain (short consensus repeat or complement control protein repeat), which is essential for interleukin 15 (IL-15) binding and IL-15Rα function, a linker/hinge region, and a membrane-proximal proline-threonine-rich region followed by a transmembrane domain, and cytoplasmic domain. IL-15Rα can homodimerize as well as heterodimerize with IL-2Rβ/CD122 and IL-2Rγ/CD132. IL-15Rα specifically binds IL-15 with very high affinity and is capable of binding IL-15 independently of other subunits. IL-15 stimulations of NK cells and T-cells has been shown to sustain long-lasting antitumor immunity, making IL-15Rα an attractive therapeutic target for immunotherapies. Mouse IL-15Rα, the murine homolog of human IL-15Rα with conserved structural domains, is a species-specific tool essential for basic research, translational research and preclinical studies.
Alternate Names: IL-15Ra, IL15Ra, cluster of differentiation 215, CD215, interleukin 15 receptor subunit alpha