Bioactive, Mouse IFNγR1 Protein Dimer, His Tag
| Product Code | CSP-25156-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for IFNγR1-specific antibody and interferon γ (IFNγ) ligand protein binding assays. SPR for IFNγR1-specific antibody and IFNγ ligand protein binding assays. |
| Suggested Applications | BLI for IFNγR1-specific antibody and IFNγ protein dimer binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | A26-S254 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized mouse IFNγR1 protein dimer, His Tag (CSP-25156-01) at 2 μg/mL (100 μL/well) can bind anti-mouse IFNγR1 monoclonal antibody with half maximal effective concentration (EC50) range of 7.4-29.6 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized mouse IFNγ at 2 μg/mL (100 μL/well) can bind IFNγR1 protein dimer, His Tag (CSP-25156-01) with half maximal effective concentration (EC50) range of 0.9-3.7 ng/mL (QC tested).
SDS-PAGE
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Mouse interferon gamma receptor 1 (IFNγR1) is a Type I integral membrane glycoprotein. The recombinant mouse IFNγR1 protein dimer (CSP-25156-01) is a cis-homodimer (cis-dimer) and contains a IFNγR1 extracellular domain (UniProt# P15261, amino acids Ala26-Ser254) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant mouse IFNγR1 protein dimer is bioactive and can bind to interferon γ (IFNγ) cytokine. It also binds IFNγR1-specific antibodies. This IFNγR1 protein dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IFNgR1
UniProt #: P15261
Predicted Molecular Weight: 68 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reduced condition is 120-190 kDa on SDS PAGE.
Protein Construct: IFNγR1 protein dimer contains a IFNγR1 extracellular domain (UniProt# P15261) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Mouse interferon gamma receptor 1 (IFNγR1), also known as cluster of differentiation 119 (CD119), IMD27A and IMD27B, is a subunit of interferon gamma receptor (IFNγR). IFNγR belongs to the type II cytokine receptor family. IFNγR1 is a Type I integral membrane glycoprotein containing extracellular, transmembrane and intracellular domains. IFNγR consists of two subunits: IFNγR1 (ligand-binding) and IFNγR2 (signal transduction). The extracellular domain has two immunoglobulin-like (Ig-like) C2-type domains. The interferon gamma (IFNγ) protein interacts with two IFNγR1 molecules to activate the cascade signaling pathway. IFNγR is a crucial component of the JAK-STAT signaling pathway that mediates the biological effects of IFN-γ in mice.
Alternate Names: cluster of differentiation 119 (CD119), IMD27A, IMD27B, IFNgR1