Bioactive, Human IL-2Rβ/γ Dimer, His-Avi Tag
| Product Code | CSP-24045-A1B5 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for IL-2Rβ-specific and IL-2Rγ-specific antibodies and Interleukin-2 (IL-2) ligand protein binding assays. |
| Suggested Applications | SPR & BLI for IL-2Rβ-specific and IL-2Rγ-specific antibodies and IL-2 protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | A27-T240 ; L23-A262 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human IL-2Rβ/γ protein heterodimer, His-Avi Tag (Cat. No. CSP-24045-A1B5) at 2 μg/mL (100 μL/well) can bind anti-human IL-2Rβ monoclonal antibody with half maximal effective concentration (EC50) range of 114.7-458.8 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human IL-2Rβ/γ protein heterodimer, His-Avi Tag (Cat. No. CSP-24045-A1B5) at 2 μg/mL (100 μL/well) can bind Interleukin-2 (IL-2) with half maximal effective concentration (EC50) range of 0.6-2.6 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: IL-2Rβ/γ heterodimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human interleukin-2 beta/gamma receptor (IL-2Rβ/γ) is a member of the type I cytokine receptor family. IL-2Rβ/γ This recombinant IL-2Rβ/γ protein (CSP-24045-A1B5) is a protein heterodimer containing IL-2Rβ subunit (UniProt# P14784, amino acids Ala27-Thr240) and IL-2Rγ subunit (UniProt# P31785 amino acids Leu23-Ala262) extracellular domains and fused with a proprietary cis-dimer motif followed by a His tag and an Avi tag at the IL-2Rβ C-terminus and IL-2Rγ C-terminus, respectively. This heterodimeric protein is expressed in HEK293T cells. The IL-2Rβ/γ protein heterodimer is bioactive and can bind to Interleukin-2 (IL-2). It also binds IL-2Rβ-specific and IL-2Rγ-specific antibodies. This IL-2Rβ/γ heterodimer can be used as an antigen for in vitro assays and antibody screening, and an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IL-2Rβ/γ
UniProt #: P14784 ; P31785
Predicted Molecular Weight: 69 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer with glycosylation under non-reduced condition is 120-190 kDa on SDS PAGE.
Protein Construct: IL-2Rβ/γ protein heterodimer contains IL-2Rβ and IL-2Rγ extracellular domains (Uniprot# P14784 and Uniprot# P31785) fused with a proprietary dimer motif followed by a His tag and an Avi tag at the IL-2Rβ C-terminus and IL-2Rγ C-terminus, respectively.
Background
Human interleukin-2 beta/gamma receptor (IL-2Rβ/γ) is a member of the type I cytokine receptor family. IL-2Rβ/γ is also known as interleukin-2 β/γ receptor, IL-2RB/IL-2RG, and Cluster of Differentiation 25 (CD25). IL-2 receptor (IL-2R) can be composed of several combinations of three proteins or “chains”: IL-2 receptor alpha (IL-2Rα), IL-2 receptor beta (IL-2Rβ), and IL-2 receptor gamma (IL-2Rγ). This IL-2Rβ/γ protein heterodimer is composed of IL-2Rβ and IL-2Rγ subunits to form the IL-2Rβ/γ heterodimer. On the cell membrane, the beta chain is complexed with Janus kinase 1 (JAK1) and the gamma chain complexes with Janus kinase 3 (JAK3); through these interactions, upon Interleukin-2 (IL-2) binding, intracellular signaling pathways are initiated. Memory-phenotype CD8+ CD44+ T cells and NK cells highly express the IL-2Rβ/γ form of the receptor. Stimulation of IL-2Rβ/γ has shown promise as an antitumor immunity booster and a recombinant protein mimicking the IL-2Rβ/γ heterodimer conformation can be crucial for therapeutic discovery.
Alternate Names: IL2R, Cluster of Differentiation 25, CD25, IL2RB, IL-2RB, Cluster of Differentiation 122, CD122, IL15RB, P70-75, interleukin 2 receptor subunit beta, IMD63, SCIDX1, IMD4, Cluster of Differentiation 132, CD132, IL2RG, CIDX, IL-2RG, P64, SCIDX, interleukin 2 receptor subunit gamma