Bioactive, Human TIGIT Dimer, Fc Tag
| Product Code | CSP-24028 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for TIGIT-specific antibody, Nectin-4 ligand protein and CD155 receptor protein binding assays. |
| Suggested Applications | SPR & BLI for TIGIT-specific antibody, Nectin-4 ligand protein and CD155 receptor protein binding assays. Animal immunization, RUO. |
| Purity | >90% dimer form as determined by SDS PAGE under non-reducing conditions. |
| Amino Acid Range | Met22-Phe141 |
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Anti-Human TIGIT Antibody Binding
Immobilized TIGIT-Fc dimer protein (Cat. No. CSP-24028) at 2 μg/mL (100 μL/well) can bind anti-human TIGIT monoclonal antibody with EC50 range of 6.27-25.07 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition NR: TIGIT dimer under non-reducing condition
Specifications
Protein Name: Human TIGIT
UniProt #: Q495A1
Predicted Molecular Weight: 104 kDa
SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing condition is 85-150 kDa on SDS PAGE.
Protein Construct: TIGIT protein dimer contains a TIGIT extracellular domain with a homodimer Fc tag at the C-terminus.
Formulation: 0.2μm filtered PBS, pH 7.4, no preservatives
Shipping: Frozen Dry Ice
Storage: -80°C
Human TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9, VSTM3. TIGIT protein dimer (CSP-24018) and contains a TIGIT extracellular domain (amino acids Met22-Phe141, UniProt# Q49A1) with a homodimer Fc tag at the C-terminus. This protein is expressed in HEK293 cells. The TIGIT-Fc dimer protein is bioactive and can bind to Nectin-4. It also binds to TIGIT-specific antibodies. This TIGIT dimer can be used as an antigen for in vitro assays and antibody screening, targeting more conformations epitopes.
Background
Human TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 (V-set and immunoglobulin domain-containing protein 9), VSTM3 (V-set and transmembrane domain-containing protein 3). TIGIT is a type I membrane protein containing an immunoglobulin variable (Ig-V) domain, a transmembrane domain and cytoplasmic domain. TIGIT is an immune receptor present on peripheral memory and regulatory CD4+ T cells and natural killer (NK) cells. TIGIT binds to CD155 (the poliovirus receptor, PVR) with high affinity and binds to CD112 (PVRL2) with lower affinity. Nectin-4 is also a ligand for TIGIT.
Alternate Names: VSIG9, VSTM3