Human Siglec-6 Protein Dimer, His Tag
| Product Code | CSP-25203-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for Siglec-6-specific antibody binding assays. |
| Suggested Applications | SPR & BLI for Siglec-6-specific antibody binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | Q27-V347 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human Siglec 6 protein dimer, His Tag (CSP-25203-01) at 2 μg/mL (100 μL/well) can bind anti-human Siglec 6 monoclonal antibody with half maximal effective concentration (EC50) range of 3.1-12.2 ng/mL (QC tested).
SDS-PAGE
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human sialic acid-binding Ig-like lectin 6 (Siglec-6) is a Type I transmembrane protein and a member of the Siglec family and immunoglobulin superfamily. The recombinant Siglec-6 protein dimer (CSP-25203-01) is a cis-homodimer (cis-dimer) and contains a Siglec-6 extracellular domain (UniProt# O43699, amino acids Gln27-Val347) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human Siglec-6 dimer protein can potently bind Siglec-6-specific antibodies. This Siglec-6 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: Siglec 6
UniProt #: O43699
Predicted Molecular Weight: 87 kDa
SDS PAGE Molecular Weight: The migration range of the protein dimer with glycosylation under non-reducing condition is 120 to 190 kDa on SDS PAGE.
Protein Construct: Siglec-6 protein dimer contains a Siglec-6 extracellular domain (UniProt# O43699) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Human sialic acid-binding Ig-like lectin 6 (Siglec-6) is a Type I transmembrane protein, a member of the Siglec family, part of the immunoglobulin superfamily, and a multifunctional immune regulator. Siglec-6 is also known as Cluster of Differentiation 327 (CD327), CD33-like (CD33L), CD33L1, CD33L2, and CDW327. Siglec-6 contains an extracellular domain with an N-terminal V-type immunoglobulin domain (Ig domain) and two C2-type Ig domains followed by a transmembrane domain and cytoplasmic signaling domain consisting of the immunoreceptor tyrosine-based inhibitory motif (ITIM). Siglec-6 is expressed on trophoblasts and highly expressed on mast cells from a variety of tissues. It binds sialylated glycans on cell surfaces or secreted proteins, modulating cell-cell interactions and immune responses. The presence of Siglec-6 on various kinds of leukemias has made it an emerging target for cancer therapeutics.
Alternate Names: Cluster of Differentiation 327, CD327, CD33-like, CD33L, CD33L1, CD33L2, CDW327, SIGLEC6, Siglec 6