Bioactive, Human Nectin-4 cis-dimer, His Tag
| Product Code | CSP-24016 |
| Expression Host | HEK293T |
| Verified Applications | ELISA, Western blot for Nectin-4-specific antibody and TIGIT receptor protein binding assays; animal immunization, RUO |
| Suggested Applications | BLI, SPR, B cell sorting |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | Gly32-Ser349 |
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Bioactivity – Antibody Binding
Immobilized human Nectin-4 dimer protein (Cat. No. CSP-24016) at 2 μg/mL (100 μL/well) can bind anti-human Nectin-4 monoclonal antibody with half maximal effective concentration (EC50) range of 1.1 – 4.3 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human Nectin-4 dimer protein (Cat. No. CSP-24016) at 5 μg/mL (100 μL/well) can bind TIGIT-His-Avi dimer protein (Cat. No. CSP-24028-03), (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: Nectin-4 dimer under non-reducing condition
Specifications
Protein Name: Human Nectin-4
UniProt #: Q96NY8
Predicted Molecular Weight: 92 kDa
SDS PAGE Molecular Weight: Migration range of the dimer under non-reducing condition is 90-130 kDa on SDS-PAGE.
Protein Construct: Nectin-4 protein cis-dimer (amino acids Gly32-Ser349) contains Nectin-4 extracellular 3 Ig-like domains (UniProt# Q96NY8) with a homodimer motif and a His tag at the C-terminus.
Background
Nectin-4, also known as PVRL-4, is a type 1 integral membrane glycoprotein that can form a homo-cis dimer on the cell membrane. It consists of an extracellular region with three immunoglobulin-like (Ig-like) domains, organized as a variable-like (Ig-V) domain followed by two constant-like (Ig-C) domains (V-C-C), one transmembrane region, and a cytoplasmic domain. Nectin-4 promotes tumor growth and is overexpressed in numerous tumor types such as breast, lung, ovarian, urothelial, colorectal, and pancreatic cancer. Nectin-4 is a ligand of TIGIT (T-cell immunoreceptor with Ig and ITIM domains). Nectin-4 is an emerging target of cancer therapeutics. A recombinant protein mimicking the Nectin-4 native dimer conformation can be crucial for therapeutic antibody and vaccine discovery.
Alternate Names: Poliovirus receptor-related-4PVRL4, LNIR, PRR4, EDSS1
Learn more about Nectin-4
Poster: Engineered novel bioactive Nectin-4 Cis-homodimer protein significantly enhances the binding of TIGIT
This novel Nectin-4 cis-dimer protein is bioactive and in the desirable conformation: (a) resulting in an increased binding potency to its receptor TIGIT and to Nectin-1, and (b) eliciting strong antibody responses in mice when using DNA immunization.
Bioactive Nectin-4 Homodimer vs Monomer – Custom Bioengineering Capabilities
What is Nectin-4? Nectin-4, a homodimer, is overexpressed on various types of tumor cells including pancreatic cancers. It has been recently identified as a novel