Bioactive, Human IL10Rα Dimer, His Tag
| Product Code | CSP-24018 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for IL10Rα-specific antibody and IL10 cytokine ligand protein binding assays. |
| Suggested Applications | SPR & BLI for IL10Rα-specific antibody and IL10 cytokine ligand protein binding assays. |
| Purity | >90% dimer form as determined by SDS PAGE under non-reducing conditions. |
| Amino Acid Range | His22-Asn235 |
For Research Use Only
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Anti-human IL10Rα Antibody Binding
Immobilized human IL10Rα dimer protein (Cat. No. CSP-24018) at 2 μg/mL (100 μL/well) can bind anti-human IL10Rα monoclonal antibody with EC50 range of 14.6-58.2 ng/mL (QC tested).
Human IL10Rα – Ligand Binding
SDS-PAGE
MW: Molecular Weight marker reduced condition NR: IL-10Rα dimer under non-reducing condition
Specifications
Protein Name: Human IL10Rα
UniProt #: Q13651
Predicted Molecular Weight: 101 kDa
SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing condition is 100-150kDa on SDS PAGE.
Protein Construct: IL10Rα protein dimer contains 2 extracellular domains with a homodimer motif and a His tag at the C-terminus.
Formulation: 0.2μm filtered PBS, pH 7.4, no preservatives
Shipping: Frozen Dry Ice
Storage: -80°C
Human IL10Rα (interleukin 10 receptor subunit alpha) is also known as CD210, IL10R, CD210α, CDW210A, and IL-10R1. IL10Rα protein dimer (CSP-24018) contains 2 IL10Rα extracellular domains (amino acids His22-Asn235, UniProt# Q13651) with a homodimer motif and a His tag at the C-terminus. This protein is expressed in HEK293 cells. The IL10Rα dimer protein is bioactive and can bind to IL10 cytokine with dramatically increased binding potency compared with the IL10Rα monomer. It also binds to IL10Rα-specific antibodies. This IL10Rα dimer can be used as an immunogen for animal immunization (RUO) and as an antigen for in vitro assays and antibody screening targeting more conformational epitopes.
Background
Interleukin-10 receptor alpha (IL10Rα), also known as IL-10 R1, CD210, CD210α, CDW210A, is a subunit of interleukin-10 receptor (IL10R) complex. IL10R belongs to the type II cytokine receptor family. IL10Rα is a type I integral membrane glycoprotein containing extracellular, transmembrane and intracellular domains. The extracellular domain has two immunoglobulin-like (Ig-like) C2-type domains. IL-10 cytokine dimer interacts with two IL10Rα molecules to activate the cascade signaling pathway.
Alternate Names: CD210, IL10R, CD210α, CDW210A, HIL-10R, and IL-10R1