Bioactive, Human IFNγR1 Dimer, His Tag
| Product Code | CSP-24015 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for IFNγR1-specific antibody and IFNγ cytokine ligand protein binding assays. |
| Suggested Applications | SPR & BLI for IFNγR1-specific antibody and IFNγ cytokine ligand protein binding assays. Animal immunization, RUO. |
| Purity | >90% dimer form as determined by SDS PAGE under non-reducing conditions. |
| Amino Acid Range | Glu18-Gly245 |
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Anti-human IFNγR1 Antibody Binding
Immobilized human IFNγR1 dimer protein (Cat. No. CSP-24015) at 2 μg/mL (100 μL/well) can bind anti-human IFNγR1 monoclonal antibody with EC50 range of 9.2-36.9 ng/mL (QC tested).
Human IFNγR1 Dimer – Ligand Binding
Immobilized human IFNγ protein at 2 μg/mL (100 μL/well) can bind IFNγR1 dimer protein (Cat. No. CSP-24015) with EC50 range of 0.95 – 3.8 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition NR: IFNγR1 dimer under non-reducing condition
Specifications
Protein Name: Human IFNγR1
UniProt #: P15260
Predicted Molecular Weight: 98 kDa
SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing condition is 90-120 kDa on SDS PAGE.
Protein Construct: IFNγR1 protein dimer contains IFNγR1 2 extracellular domains with a homodimer motif and a His tag at the C-terminus.
Formulation: 0.2μm filtered PBS, pH 7.4, no preservatives
Shipping: Frozen Dry Ice
Storage: -80°C
Human IFNγR1 (interferon gamma receptor 1) is also known as CD119, IFNγR, IMD27A, and IMD27B. IFNγR1 protein dimer (CSP-24015) contains IFNγR1 2 extracellular domains (amino acids Glu18-Gly245, UniProt# P15260) with a homodimer motif and a His tag at the C- terminus. This protein is expressed in HEK293 cells. The IFNγR1 dimer protein is bioactive and can bind to IFNγ cytokine with dramatically increased binding potency compared to the IFNγR1 monomer. It also binds to IFNγR1-specific antibodies. This IFNγR1 dimer can be used as an immunogen for animal immunization (RUO) and as an antigen for in vitro assays and antibody screening targeting more conformational epitopes.
Background
Interferon gamma receptor 1 (IFNγR1), also known as CD119, IMD27A and IMD27B, is a subunit of interferon gamma receptor (IFNγR). IFNγR belongs to the type II cytokine receptor family. IFNγR1 is a type I integral membrane glycoprotein containing extracellular, transmembrane and intracellular domains. The extracellular domain has two immunoglobulin-like (Ig-like) C2-type domains. The interferon gamma (IFNγ) dimer interacts with two IFNγR1 molecules to activate the cascade signaling pathway.
Alternate Names: CD119, IFNγR, IMD27A, and IMD27B
Learn more about IFNγR1
IFNγR1 Dimeric Protein Enhances Receptor/Ligand Binding
Interferon Gamma Receptor 1 (IFNγR1) is a critical component of the immune system’s response to pathogens and cancer. This protein is essential for recognizing and