Bioactive, Recombinant Human FGFR4 Protein Dimer, His-Avi Tag
| Product Code | CSP-25132-03 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for FGFR4-specific antibody binding assay and BLI for fibroblast growth factor 1 (FGF-1) ligand protein binding assays. |
| Suggested Applications | SPR for FGFR4-specific antibody and FGF-1 protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | L22-D369 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human FGFR4-His-Avi protein dimer (CSP-25132-03) at 2 μg/mL (100 μL/well) can bind anti-human FGFR4 polyclonal antibody with half maximal effective concentration (EC50) range of 2.4-9.7 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: FGFR4 dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human fibroblast growth factor receptor 4 (FGFR4) is a cell surface receptor belonging to the immunoglobulin superfamily functioning as a receptor tyrosine kinase (RTK). FGFR1 can form ligand-dependent and ligand-independent dimers. The recombinant FGFR4 protein dimer (CSP-25132-03) is a cis-homodimer (cis-dimer) and contains a FGFR4 extracellular domain (UniProt# P22455, amino acids Leu22-Asp369) fused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human FGFR4 protein dimer is bioactive and can bind to fibroblast growth factor 1 (FGF-1). It also binds FGFR4-specific antibodies. This FGFR4 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: FGFR4
UniProt #: P22455
Predicted Molecular Weight: 97 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is ~190 kDa on SDS PAGE.
Protein Construct: FGFR4 dimer protein contains a FGFR4 extracellular domain (UniProt# P22455) fused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus.
Background
Human fibroblast growth factor receptor 4 (FGFR4) is a cell surface receptor belonging to the immunoglobulin superfamily and a transmembrane receptor tyrosine kinase (RTK) that belongs to the FGFR family. FGFR4 is also known as cluster of differentiation 334 (CD334), JTK2, and TKF. FGFR4 contains an extracellular domain with three immunoglobulin-like (Ig-like) subdomains (D1, D2 and D3), followed by a transmembrane, and an intracellular domain. Dimerization of FGFRs is necessary for activation and they can homodimerize and heterodimerize in both the presence and absence of ligand. FGFRs bind fibroblast growth factors (FGFs) leading to phosphorylation and triggering signaling cascades. FGFR4 has been implicated in oncogenesis, tumor progression, and resistance to anti-tumor therapy in multiple types of cancer making it an emerging target for cancer therapeutics.
Alternate Names: FGFR-4, cluster of differentiation 334, CD334, JTK2, TKF