Bioactive, Human FGFR1 Dimer, His-Avi Tag
| Product Code | CSP-25129-03 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for FGFR1-specific antibody and BLI for fibroblast growth factor 1 (FGF-1) ligand protein binding assays. |
| Suggested Applications | SPR for FGFR1-specific antibody and FGF-1 protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | R22-E376 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human FGFR1-His-Avi dimer protein (CSP-25129-03) at 2 μg/mL (100 μL/well) can bind anti-human FGFR1 monoclonal antibody with half maximal effective concentration (EC50) range of 10.1-40.4 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: FGFR1 dimer under non-reduced condition
Specifications
Formulation: 0.2μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human fibroblast growth factor receptor 1 (FGFR1) is a cell surface receptor belonging to the immunoglobulin superfamily, functioning as a receptor tyrosine kinase (RTK). FGFR1 can form a ligand-dependent and ligand-independent dimer. The recombinant FGFR1 dimer protein (CSP-25129-03) is a cis-homodimer (cis-dimer) and contains a FGFR1 extracellular domain (UniProt# P11362, amino acids Arg22-Glu376) fused with a proprietary dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293 cells. The recombinant human FGFR1 dimer protein is bioactive and can bind to fibroblast growth factor 1 (FGF-1). It also binds FGFR1-specific antibodies. This FGFR1 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: FGFR1
UniProt #: P11362
Predicted Molecular Weight: 99 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is ~190 kDa on SDS PAGE.
Protein Construct: FGFR1 dimer protein contains a FGFR1 extracellular domain (UniProt# P11362) fused with a proprietary dimer motif followed by a tandem His-Avi tag at the C-terminus.
Background
Human fibroblast growth factor receptor 1 (FGFR1) is a cell surface receptor belonging to the immunoglobulin superfamily and a transmembrane receptor tyrosine kinase (RTK) that belongs to the FGFR family. FGFR1 is also known as basic fibroblast growth factor receptor 1 (BFGFR), cluster of differentiation 331 (CD331), CEK, FGFBR, FLG, Fms-like tyrosine kinase 2 (FLT-2), HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, and ECCL. FGFR1, a Type I transmembrane protein, contains an extracellular domain with three immunoglobulin-like (Ig-like) subdomains (D1, D2 and D3), followed by a transmembrane, and an intracellular domain. Dimerization of FGFRs is necessary for activation and they can homodimerize and heterodimerize in both the presence and absence of ligand. FGFRs bind fibroblast growth factors (FGFs) leading to phosphorylation and triggering signaling cascades. FGFR1 overexpression has been reported in a variety of cancers including up to 22% of non-small-cell lung carcinoma, 14% of urinary bladder transitional cell carcinomas, 10% of estrogen receptor positive breast cancers, and 10% of squamous cell head and neck cancers. Ligand-independent FGFR1 dimerization is an important driver of hematologic malignancies, solid tumors, and developmental disorders. Inhibition of FGFRs has been an emerging target for cancer therapeutics, therefore, a recombinant protein mimicking the FGFR1 dimer conformation can be critical for cancer therapeutic discovery.
Alternate Names: FGFR-1, basic fibroblast growth factor receptor 1, BFGFR, cluster of differentiation 331, CD331, CEK, FGFBR, FLG, Fms-like tyrosine kinase 2, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1, ECCL