Bioactive, Human CD4 Dimer, His tag
| Product Code | CSP-24004 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for antibody binding and gp120 binding. |
| Suggested Applications | SPR binding measurements, B cell sorting, immunogen for animal immunization, RUO. |
| Purity | >90% dimer form as determined by SDS-PAGE non-reducing conditions |
| Amino Acid Range | Lys26-Phe396 |
For Research Use Only (RUO)
Price: 550.00
Price: $900.00
Price: $1,150.00
Price: $2,200.00
Price: $7,040.00
Anti-human CD4 Antibody Binding
Antibody binding (EC50 range of 3.46 – 13.85 ng/mL)
gp120 Ligand Binding
HIV-1 gp120 ligand binding (EC50 range of 11.45 – 45.8 ng/mL)
SDS-PAGE
NR: CD4 dimer under non-reducing condition
Specifications
Formulation: 0.2μm filtered PBS, pH 7.4, no preservatives
Shipping: Frozen Dry Ice
Storage: -80C
Human CD4 protein is also known as IL16 receptor (IL16R). CD4 dimer (CSP-24004) contains CD4 extracellular 4-domains (amino acids Lys26- Phe396, UniProt# A0A4Y5UGE4) with a homodimer motif and a His tag at the C-terminus. This protein is expressed in HEK293 cells. The CD4 dimer protein is bioactive and can bind to HIV-1 envelope glycoproteins (Env) from various subtypes with on average >10-fold binding activity increases compared to CD4 monomer protein. The HIV-1 Env binding to CD4 dimer can be diminished by a D368R mutation. CD4 dimer also increases the binding to CD4-specific antibodies. This CD4 dimer can be used as an immunogen for animal immunization (RUO) and as an antigen for in vitro assays and antibody screening targeting more conformations epitopes.
Protein Name: Human CD4
UniProt #: A0A4Y5UGE4
Predicted Molecular Weight: 112 kDa
SDS PAGE Molecular Weight: Migration range of the dimer under non-reducing condition is 100-150kDa on SDS PAGE
Protein Construct: CD4 dimer contains CD4 extracellular 4-domains with a homodimer motif and a His tag at the C-terminus.
Background
CD4 is type 1 integral membrane glycoprotein protein on a T cell surface, also known as known as T-cell surface antigen T4/Leu-3. CD4 contains an extracellular domain, a transmembrane domain and a cytoplasmic domain. The extracellular domain has 4 immunoglobulin-like (Ig-like) domains: one Ig-like V-type domain and three Ig-like C2-type domains. The CD4 extracellular domain is responsible for MHC class-II antigen/T-cell receptor interaction and T cell activation. CD4 is also known as interleukin 16 receptor (IL16R). The IL16 cytokine binds CD4 to activate a downstream signalling cascade. CD4 is also the primary receptor for the human immunodeficiency virus (HIV) envelope glycoprotein gp120 to mediate HIV infection and entry into host T cells, as the underlying cause of acquired immune deficiency syndrome (AIDS).
Alternate Names: IL16R