Bioactive, Human EpoR Dimer, His-Avi Tag
| Product Code | CSP-24087 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for EpoR specific antibody and erythropoietin (Epo) ligand protein binding assays. |
| Suggested Applications | ELISA for EpoR-specific antibody and erythropoietin (Epo) ligand protein binding assays. SPR & BLI for EpoR-specific antibody and Epo protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | A25-P250 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human EpoR-His-Avi dimer protein (CSP-24087) at 2 μg/mL (100 μL/well) can bind anti-human EpoR monoclonal antibody with half maximal effective concentration (EC50) range of 9.1-36.2 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human Epo at 2 μg/mL (100 μL/well) can bind EpoR-His-Avi dimer protein (CSP-24087) with half maximal effective concentration (EC50) range of 0.3-1.2 μg/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: EpoR dimer under non-reducing condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human erythropoietin receptor (EpoR) is a transmembrane protein found on the surface of erythropoietin (Epo) responding cells and functions as a homodimer. The recombinant EpoR dimer protein (CSP-24087) is a cis-homodimer (cis-dimer) and contains an EpoR extracellular domain (UniProt# P19235, amino acids Ala25-Pro250) fused with a proprietary dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human EpoR dimer protein is bioactive and can bind to erythropoietin (Epo). It also binds EpoR-specific antibodies. This EpoR dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: EpoR
UniProt #: P19235
Predicted Molecular Weight: 70 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing conditions is 115-125 KDa on SDS PAGE.
Protein Construct: EpoR dimer protein contains an EpoR extracellular domain (UniProt# P19235) fused with a proprietary dimer motif followed by a tandem His-Avi tag at the C-terminus.
Background
Human erythropoietin receptor (EpoR), also known as EPO-R and CD131, is a transmembrane protein found on the surface of erythropoietin (Epo) responding cells. EpoR belongs to the type I cytokine receptor family. EpoR contains an extracellular domain with two fibronectin type II domains, a transmembrane domain, a juxtamembrane region, and a cytoplasmic region. It functions as a homodimer to form an active receptor complex upon ligand binding that is crucial for receptor activation and downstream signaling. EpoR dysregulation is involved in blood disorders including the development of erythroleukemia and familial erythrocytosis. Therefore, a recombinant protein mimicking the EpoR dimer conformation can be crucial for therapeutic discovery.
Alternate Names: EPOR, EPO-R, CD131