Bioactive, Human CTLA-4 Dimer, His Tag
| Product Code | CSP-24031 |
| Expression Host | HEK293T |
| Verified Applications | ELISA, Western blot for CTLA-4-specific antibody and CD80 ligand protein binding assays. BLI protein binding assays for CTLA-4 with CD80. |
| Suggested Applications | SPR & BLI for CTLA-4-specific antibody or CD80 ligand protein binding assays. Western blot with CTLA-4-specific antibody. ELISA for CTLA-4-specific antibody or CD80 ligand protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | Lys36-Asp161 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human CTLA-4 dimer protein, His Tag (Cat. No. CSP-24031) at 2 μg/mL (100 μL/well) can bind anti-human CTLA-4 monoclonal antibody with half maximal effective concentration (EC50) range of 10.5-42 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human CTLA-4 dimer protein, His Tag (Cat. No. CSP-24031) at 2 μg/mL (100 μL/well) can bind human CD80 dimer protein, with half maximal effective concentration (EC50) range of 0.5-2 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: CTLA-4 dimer under non-reducing condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Cytotoxic T-lymphocyte associated protein 4 (CTLA-4) is also known as CD152 (cluster of differentiation 152), CD152 antigen, CTLA4, ALPS5, CD, CELIAC3, GRD4, GSE, and IDDM12. The CTLA-4 dimer protein (CTLA-4 protein dimer, CSP-24031) is a cis-homodimer (cis-dimer) and contains the CTLA-4 extracellular domain (UniProt# P16410, amino acids Lys36-Asp161) fused with a proprietary dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293 cells. The CTLA-4 dimer protein is bioactive and can enhance the binding to CD80. It also binds CTLA-4-specific antibodies. This recombinant CTLA-4 dimer protein can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: CTLA-4
UniProt #: P16410
Predicted Molecular Weight: 50 kDa
SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing conditions is 50-60 kDa on SDS PAGE.
Protein Construct: CTLA-4 protein dimer contains a CTLA-4 extracellular domain (Uniprot# P16410) fused with a proprietary dimer motif followed by a His tag at the C-terminus.
Background
Cytotoxic T-lymphocyte associated protein 4 (CTLA-4), also known as CD152 (cluster of differentiation 152), CD152 antigen, CTLA4, ALPS5, CD, CELIAC3, GRD4, GSE, and IDDM12 is a member of the immunoglobulin superfamily. CTLA-4 contains an extracellular immunoglobulin-like (Ig-like) domain (an Ig-V-like and an Ig-C-like domain), a transmembrane domain, and a cytoplasmic tail. As an immune checkpoint CTLA-4 binds both CD80 (Cluster of differentiation 80) and CD86 (Cluster of differentiation 86) to transmit an inhibitory signal to T cells, competing with CD28 (Cluster of differentiation 28) which transmits a stimulatory signal. It is often overexpressed in malignancies caused by immunosurveillance, making the inhibition of immune checkpoint proteins like CTLA-4 an emerging strategy in cancer therapy. CTLA-4 gene variants have been associated with Type 1 diabetes, Graves’ disease, Hashimoto’s thyroiditis, celiac disease, and other autoimmune diseases.
Alternate Names:Cytotoxic T-lymphocyte associated protein 4, CTLA4, CD152, CD152 antigen, ALPS5, CD, CELIAC3, GRD4, GSE, IDDM12