Bioactive, Human CD80 Dimer, Flag-His Tag
| Product Code | CSP-24033-02 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for CD80-specific antibody and CTLA-4 or CD28 ligand protein binding assays. |
| Suggested Applications | ELISA for CD80-specific antibody and CTLA-4 or CD28 ligand protein binding assays. SPR & BLI for CD80-specific antibody and CTLA-4 or CD28 protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | Val35-Asn242 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized CD80-Flag-His dimer protein (Cat. No. CSP-24033-02) at 2 μg/mL (100 μL/well) can bind anti-human CD80 monoclonal antibody with half maximal effective concentration (EC50) range of 4.9-19.6 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human CTLA-4 dimer protein, His Tag (Cat. No. CSP-24031) at 2 μg/mL (100 μL/well) can bind human CD80-Flag-His (Cat. No. CSP-24033-02) dimer protein, with half maximal effective concentration (EC50) range of 0.4-1.5 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: CD80 dimer under non-reducing condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human CD80 (Cluster of differentiation 80) is a type I transmembrane glycoprotein in the immunoglobulin superfamily and is a member of the B7 Family of ligands. CD80 exists as a monomer but its dimeric form can influence immune regulation and contribute to pathogenic conditions. The recombinant CD80 dimer protein (CSP-24033-02) is a cis-homodimer (cis-dimer) and contains a CD80 extracellular domain (UniProt# P33681, amino acids Val35-Asn242) fused with a proprietary dimer motif followed by a tandem Flag-His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant CD80 dimer protein is bioactive and can bind to CTLA-4 or CD28. It also binds CD80-specific antibodies. This CD80 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: CD80
UniProt #: P33681
Predicted Molecular Weight: 66 kDa
SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing conditions is 100-170 kDa on SDS PAGE.
Protein Construct: CD80 dimer protein contains a CD80 extracellular domain (UniProt# P33681) fused with a proprietary dimer motif followed by a tandem Flag-His tag at the C-terminus.
Background
Human CD80 (Cluster of differentiation 80) is a type I transmembrane glycoprotein in the immunoglobulin superfamily and is a member of the B7 Family of ligands. CD80 is also known as B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, and LAB7. CD80 contains an extracellular domain (ECD), a transmembrane domain, and a cytoplasmic domain. The ECD consists of two immunoglobulin (Ig)-like subdomains, a variable-like domain (Ig-V-like domain), and a constant-like domain (Ig-C-like domain). It is primarily expressed on antigen-presenting cells (APCs), such as dendritic cells, macrophages, and B cells. CD80 interacts with CTLA-4 (Cytotoxic T-lymphocyte associated protein 4) to transmit an inhibitory signal with T cells and with CD28 (Cluster of differentiation 28) to transmit a stimulatory signal. It is often overexpressed in various autoimmune diseases such as multiple sclerosis and systemic lupus erythematosus, as well as some cancers. CD80 exists as a monomer but its dimeric form can influence immune regulation and contribute to pathogenic conditions. A recombinant protein mimicking the CD80 dimer conformation can be crucial for therapeutic discovery.
Alternate Names: B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7