Bioactive, Human CD28 Dimer, His Tag
| Product Code | CSP-24032 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for CD28-specific antibody and CD80 ligand protein binding assays. BLI protein binding assays for CD28 with CD80. |
| Suggested Applications | ELISA for CD28-specific antibody and CD80 ligand protein binding assays. SPR & BLI for CD28-specific antibody and CD80 protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | Asn19-Pro152 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human CD28 dimer protein, His Tag (Cat. No. CSP-24032) at 2 μg/mL (100 μL/well) can bind anti-human CD28 monoclonal antibody, with half maximal effective concentration (EC50) range of 1.1-4.3 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human CD28 dimer protein, His Tag (Cat. No. CSP-24032) at 2 μg/mL (100 μL/well) can bind human CD80 dimer protein (Cat. No. CSP-24033-04), with half maximal effective concentration (EC50) range of 2.9-11.7 μg/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: CD28 dimer under non-reducing condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80C
Human CD28 (Cluster of Differentiation 28) is a type I membrane protein in the immunoglobulin superfamily and a member of the B7 Family of ligands. It exists as a homodimer on the cell surface forming a stable structure composed of two identical CD28 protein subunits. The recombinant CD28 dimer protein is a cis-homodimer (cis-dimer) protein (CSP-24032) and contains a CD28 extracellular domain (UniProt# P10747, amino acids Asn19-Pro152) fused with a proprietary dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The CD28 dimer protein is bioactive and can enhance the binding to CD80. It also binds CD28-specific antibodies. This CD28 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: Human CD28
UniProt #: P10747
Predicted Molecular Weight: 46 kDa
SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing condition is 60-120 kDa on SDS PAGE.
Protein Construct: CD28 dimer protein contains a CD28 extracellular domain (Uniprot# P10747) fused with a proprietary dimer motif followed by a His tag at the C-terminus.
Background
Human CD28 (Cluster of Differentiation 28) is a type I membrane protein is in the immunoglobulin superfamily and is a member of the B7 Family of ligands. CD28 is also known as Tp44, TP-44, T-cell-specific surface glycoprotein CD28, and CD28 Antigen. CD28 is the only B7 receptor consistently expressed on naive T cells. CD28 contains a single immunoglobulin variable-region-like (IgV-like) domain, a transmembrane domain, and a cytoplasmic domain. It exists as a homodimer on the cell surface forming a stable structure composed of two identical CD28 protein subunits. The intracellular domain contains two proline-rich motifs and a YMNM motif that are critical for effective signaling. As an immune checkpoint CD28 binds both CD80 (Cluster of differentiation 80) and CD86 (Cluster of differentiation 86) to transmit a stimulatory signal with T cells, competing with CTLA-4 (Cytotoxic T-lymphocyte associated protein 4) which transmits an inhibitory signal. CD28, as a key co-stimulatory receptor in T cell activation, is associated with several diseases, particularly those related to immune system dysfunction, including autoimmune diseases, cancers, and allergic and inflammatory disorders. CD28 is associated with autoimmune diseases including type 1 diabetes, lupus nephritis, asthma, and Crohn’s disease. The importance of CD28 signals in T-cell function makes this molecule an exciting target for drug discoveries to modulate the function of both effector T cells and Treg cells.
Alternate Names:Tp44, TP-44, T-cell-specific surface glycoprotein CD28, CD28 Antigen