Bioactive, Human 2Ig-B7H3 Dimer, His-Avi Tag
| Product Code | CSP-240982lg |
| Expression Host | HEK293T |
| Verified Applications | ELISA for 2Ig-B7H3-specific antibody binding assay. |
| Suggested Applications | ELISA for 2Ig-B7H3-specific antibody binding assays. SPR & BLI for 2Ig-B7H3-specific antibody binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | L29-A466 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human 2Ig-B7H3-His-Avi dimer protein (CSP-240982lg) at 2 μg/mL (100 μL/well) can bind anti-human B7H3 monoclonal antibody with half maximal effective concentration (EC50) range of 8.7-34.8 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: 2Ig-B7H3 dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human B7 Homolog 3 (B7H3) isoform 2Ig-B7H3 (2Ig-B7-H3, 2IgB7H3) is a Type I membrane protein characterized by an extracellular domain containing a single pair of IgV-like and IgC-like immunoglobulin domains rather than two pairs like isoform 4Ig-B7H3 (4Ig-B7-H3, 4IgB7H3). B7H3 can form a dimer that is important for its function. The recombinant 2Ig-B7H3 dimer protein (CSP-240982lg) is a cis-homodimer (cis-dimer) and contains a 2Ig-B7H3 extracellular domain with a single pair of IgV-like and IgC-like immunoglobulin domains (UniProt# Q5ZPR3-2, amino acids Leu29-Ala466) fused with a proprietary dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human 2Ig-B7H3 dimer protein is bioactive and can bind 2Ig-B7H3-specific antibodies. This 2Ig-B7H3 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: B7H3
UniProt #: Q5ZPR3-2
Predicted Molecular Weight: 67 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing conditions is 85-125 kDa on SDS PAGE.
Protein Construct: 2Ig-B7H3 dimer protein contains a 2Ig-B7H3 extracellular domain with a single pair of IgV-like and IgC-like immunoglobulin domains (UniProt# Q5ZPR3-2) fused with a dimer motif followed by a tandem His-Avi tag at the C-terminus.
Background
Human B7 Homolog 3 (B7H3, B7-H3) is a member of the B7 family of immune checkpoint proteins involved in regulating immune responses. Human B7H3 has 2 isoforms: 2Ig-B7H3 (2Ig-B7-H3, 2IgB7H3) and 4Ig-B7H3 (4Ig-B7-H3, 4IgB7H3). B7H3 is a Type I membrane protein characterized by an extracellular domain, a transmembrane domain and a cytoplasmic domain. The 2Ig-B7H3 isoform contains a single pair of IgV-like and IgC-like immunoglobulin domains while the 4Ig-B7H3 isoform has two pairs of IgV-like and IgC-like immunoglobulin domains in the extracellular region. B7H3 is also known as B7-H3, Cluster of Differentiation 276 (CD276), and B7RP-2. B7H3 has limited expression on normal tissues but is highly expressed in many cancer cells including lung, kidney, ovarian, colorectal, liver, and breast cancers, which can contribute to immune evasion by inhibiting T cell activation. B7H3 can form a dimer that is critical for its function and can more effectively interact with its receptor(s) on T cells and other immune cells. B7H3 is overexpressed in up to 60% of all cancers and due to its selective expression on solid tumors B7H3 has become a critical target for cancer therapies.
Alternate Names: Cluster of Differentiation 276 (CD276), 2IgB7-H3, 2Ig-B7H3, B7-H3, B7H3, B7RP-2