Advanced Protein Engineering Services
At Conigen, we specialize in the custom bioengineering and production of advanced proteins, using two different technology platforms:
- The CSP™ (Conigen Soluble Proteins) technology platform consists of soluble recombinant dimeric and trimeric proteins with a quaternary structure mimicking the native conformation of protein complexes on cell surfaces, such as receptors or cancer target proteins
- The CMP™ nanoparticle technology platform produces native conformational membrane proteins by using the self-assembling and then budding functions of the viral core proteins
With our proprietary advanced protein development CSP and CMP technology platforms, Conigen is equipped to meet your specific needs. Our expertise shines in creating proteins that are:
- Soluble multimeric proteins, closely representing the conformation of native receptors
- Faithfully mimicking the native conformation or native structures on nanoparticles or soluble proteins
- Highly immunogenic
- Optimized for higher target signal with minimal background
Example Targets and Applications
Targets
- Cytokine receptors
- Immune-oncology targets
- Immunology: receptors and ligands
- Type I & II membrane proteins
- Complex multipass membrane proteins
Applications
- Bioactivity assays
- In vitro stimulation or binding assay
- In vivo immunization (immunogen)
- Immunogenicity determination
- Antibody screening and characterization
Custom Services Workflow
Milestone 1: Assessment
Dedicated consultation with your scientists
Define the details of your protein and application
Transfer starting material
Milestone 2: Development
Collaborative experienced team
Conigen selects the protein platform most suited to your custom protein
Milestone 3: Testing and Delivery
Focused on quality
Purity and bioactivity assured
Deliverables:
- Large-scale protein batch
- Bioactivity reports
FAQs
How much purified protein will I receive?
In general, 1-10 mg of protein can be provided upon request.
Can I request a specific method to verify and quantify the expression of my protein variants?
Yes
Do you offer mutations other than single amino acid substitutions?
Yes, it will be based on specific request.
Can I request expression of more than one protein at a time?
Yes
What other conjugation tags are available, apart from HIS on the Fc domain?
Yes, Avitag, Biotin, Flag tag, HA tag, and Myc tag are also available in addition to His and Fc tags
What cell lines do you use for protein expression? Can I request a specific cell line?
HEK293 and CHO cell lines are commonly used. A specific cell can be used per request.
What information is included on the datasheet for these custom proteins?
Protein sequence information, purity, concentration, amount, other information upon request for each specific protein.
Do you offer assay development or other specialized R&D work?
Yes
What happens if you run into technical difficulties with my target protein and cannot complete the project?
If technical difficulties are encountered, we will inform the customer, troubleshoot, discuss with the customer and find the specific solution.