Bioactive, Recombinant Human ObR Protein Dimer, His-Avi-Tag
| Product Code | CSP-24090 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for ObR-specific antibody and leptin ligand protein binding assays. |
| Suggested Applications | ELISA for ObR-specific antibody and leptin ligand protein binding assays. SPR & BLI for ObR-specific antibody and leptin protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | F22-D839 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human ObR-His-Avi protein dimer (CSP-24090) at 2 μg/mL (100 μL/well) can bind anti-human ObR monoclonal antibody with half maximal effective concentration (EC50) range of 16.8-67.2 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human Leptin at 2 μg/mL (100 μL/well) can bind human ObR-His-Avi protein dimer (CSP-24090) with half maximal effective concentration (EC50) range of 0.1-0.5 μg/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: ObR dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human obesity receptor (ObR) is a Type I transmembrane receptor belonging to the cytokine receptor family. ObR functions as a dimer to mediate leptin signaling. The recombinant ObR protein dimer (CSP-24090) is a cis-homodimer (cis-dimer) and contains an ObR extracellular domain (UniProt# P48357, amino acids Phe22-Asp839) fused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human ObR protein dimer is bioactive and can bind to leptin. It also binds ObR-specific antibodies. This ObR dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: ObR
UniProt #: P48357
Predicted Molecular Weight: 207 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing conditions is >190 KDa on SDS PAGE.
Protein Construct: ObR protein dimer contains an ObR extracellular domain (UniProt# P48357) fused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus.
Background
Human obesity receptor (ObR) is a Type I transmembrane receptor belonging to the cytokine receptor family. ObR is the receptor of leptin, a hormone secreted by fat cells that plays a crucial role in regulating appetite, metabolism, and energy homeostasis. ObR is also known as OB-R, leptin receptor (LEPR), LEPRD, and cluster of differentiation 295 (CD295). ObR contains an extracellular domain with an N-terminal cytokine receptor homology domain (CRH-1); an immunoglobulin-like (Ig-like) domain; a second CRH domain (CRH-2), also referred to as the leptin-binding domain (LBD); and two fibronectin type III (FNIII) domains followed by a transmembrane domain and cytoplasmic domain. ObR functions as a dimer to mediate leptin signaling. ObR binds leptin and its activity regulates adipose-tissue mass making ObR an attractive target when it comes to combating obesity.
Alternate Names: OB-R, leptin receptor, LEPR, LEP-R, LEPRD, cluster of differentiation 295, CD295