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CSP™ – Conigen soluble proteins are the dimeric and trimeric extracellular domains of cell membrane proteins.
These recombinant molecules have a quaternary structure mimicking the native conformation of cell surface receptors and targets.
Proprietary dimeric or trimeric interface elements are fused with the extracellular domain of type 1 and type 2 membrane proteins with or without a tag.
The soluble proteins are expressed in mammalian cells which have similar post-translational modification processes to their natural expressing cells.
As an immunogen: presents better conformational epitopes as compared with monomeric molecules, to elicit antibodies for translational research and therapeutic discovery.
As an antigen: provides better bioactivity for in vitro assays.
Class of proteins: cytokine receptors, immune-oncology targets, immune checkpoints, B and T cell regulatory molecules.
Examples: CD4 dimers and Nectin-4 dimers
CMP™ – Conigen membrane protein nanoparticles consist of target integral membrane proteins embedded in a lipid bilayer surrounding a viral protein core.
The CMP nanoparticle technology platform produces native conformational membrane proteins by using the self-assembling and then budding functions of the viral structural proteins.
A proprietary design is used to select viral backbones with structural proteins resulting in high quality membrane protein presenting nanoparticles.
As an immunogen: generates antibodies against complex membrane protein for translational research and therapeutic discovery.
As a lipid-bilayer-based antigen: can screen for binders and analyze affinities or perform biophysical and structural investigation of complex membrane proteins.
Categories of complex membrane proteins can be expressed using the CMP technology platform: GPCRs, ion channels, immuno-oncology targets, viral envelopes.
Protein engineering services to custom build your own advanced proteins, ensuring the highest purity and bioactivity, ideal for both in vitro assays and in vivo immunization applications.
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