Engineering Conformational Proteins to Advance Discovery of Next Generation Therapeutics

Using rational protein design and multiple protein display platforms, Conigen scientists leverage modern protein engineering, molecular biology, vaccinology and immunology to provide conformational protein tools and bioengineering services for drug discovery against complex targets.

Recombinant proteins designed with quaternary structure and conformational epitopes

Conigen Soluble Dimeric Recombinant Proteins
Powered by the CSP™ Technology Platform

These bioactive homodimer and heterodimer proteins are designed to mimic native protein complexes with the ectodomain of a Type I transmembrane protein genetically fused to a proprietary cis-dimer motif with a stabilization element at the C-terminus to promote soluble dimer formation.

Applications include:

  • Tool-molecules – immunogen / antigen, antibody generation, screening, characterization, protein engineering

  • Alternative to live cell systems

  • Receptor-ligand interactions – ELISA, flow cytometry, SPR, BLI, and other binding potency methods

  • Drug screening and bioactivity evaluation with greater sensitivity and wider screening window

  • Agonist: drug binding ability to receptor

  • Antagonist: drug blocking receptor/ligand binding

  • Structural analyses

Examples: CD4 homodimer and INFa R1/R2 heterodimer

Full length multi-pass target proteins embedded in lipid bilayer of virus-like nanoparticles (VLPs)

Conigen Membrane Protein Nanoparticles
Powered by the CMP™ Technology Platform

These bioactive full-length target membrane proteins are optimized to enhance surface expression on VLPs. The viral capsid backbone is engineered to increase VLP release for higher production yield.

Applications include:

  • Immunogen generates better antibodies targeting conformational epitopes
  • Antigen to screen and characterize antibodies
  • Drug screening and bioactivity evaluation with greater sensitivity and wider screening window
  • Agonist: drug binding ability to receptor
  • Antagonist: drug blocking receptor/ligand binding
  • Structural analyses

Examples: Claudin 18.2 and CXCR4

Advanced Protein Engineering Services icon

Custom Recombinant Proteins & Antibodies

Scalable Standardized Procedures to Ensure Consistent Product Quality
From difficult to expression proteins to immunogenicity challenges, our team supports custom projects for protein and antibody generation with a modular, data-backed workflow.

  • Custom design and production of conformational soluble or membrane proteins
  • Recombinant antibody expression in HEK293 cells
  • Mouse monoclonal antibody development

Over 25 years of vaccine and antibody research and development experience, the Conigen team is driven to deliver the highest quality advanced proteins

  • No need for developing corresponding cell line for every target
  • CSP can preserve the native conformations similar to those on cell surfaces
  • Easy to scale-up and develop high-through-put assays
  • No host cell proteins
  • Dramatically higher number of molecules for each immunization
  • Highly immunogenic
  • Preserves the native conformations similar to those on cell surfaces
  • CSP not limited to monomeric proteins without critical conformational epitopes on the quaternary structures
  • Provides better conformational epitopes for antibody screening and affinity evaluation
  • Enhances assay sensitivity and potency; generates wider windows for agonist and antagonist activity evaluation
  • Better conformation for antibody/antigen, receptor/ligand complex structural analysis
  • More natively folded proteins in the cellular membrane bilayer without further processing
  • Eliciting antibodies that better target conformational epitopes
  • Higher antigen dosage per immunization
  • Reduced number of host cell derived proteins
  • More immunogenic